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2 Add 10-20 μl of the protein extract to 1 ml of the diluted reagent and mix. Accurate measurement of protein concentration is critical since the. Also kits available for protein determination from Sigma, BioRad, and. 3 The Bicinchoninic Acid BCA Assay for Protein Manal. 6 The Nitric Acid Method for Protein Estimation in Biological Samples. Scott A. The Bio-Rad DC Protein Assay is a colorimetric assay for protein concen. Peterson, Gary L, Review of the Folin Phenol Protein Quantitation Method of.
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